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A4268
Sigma
α-Amylase from porcine pancreas
Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280 )
| Enzyme Commission (EC) Number: | 3.2.1.1 ( BRENDA | IUBMB ) |
| MDL number: | MFCD00081319 |
Description
| Other Notes | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer |
| Preparation Note | 2× crystallized |
| Unit Definition | One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C. |
| Physical form | Suspension in 2.9 M NaCl solution containing 3 mM CaCl2. |
| General description | Molecular Weight: 51-54 kDa. α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of approximately 475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7. |
Properties
| type | Type I-A |
| form | saline suspension |
| EQP level | Premium |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22-45 |
| WGK Germany | 3 |
